so it is impossible to use single power supply !!

The datasheet for the AD620B presents a circuit using a single 0-5V supply. The trick is that the input signals can't be too close to either end of the supply (IIRC, circa 1.2V within either end: so for 0-5V supply, the -IN is 1.2 and the +IN is 3.8 - or thereabouts)

There are inverter ICs that can produce your negative voltage rail - the Intersil ICL7660 is one such IC, which can either double a voltage or invert (It is a lot more efficient at inverting, whereas doubling involves a couple of diode drops: 2Vin - 2Vf)

 

Forget all this, search for component HX711 (24-bit ADC for load cell, can be used in other applications as well)

Just be careful when buying the module with ground shield, there is another model that .

 

so it is impossible to use single power supply !!

is there other instrumentation amplifiers work with single power supply ?

i have tried v+=5,V-=-5 ,Vref=0.

it worked fine..

i need to use microcontroller to read the the output of ad620 .
should i need to add other elements?or connect it directly?

Why didn't you look at the datasheet of the AD620? Figure 38 on page 14 shows it in a circuit with a single 5V positive supply. It does it by biasing the pin 5 REF at half the supply voltage like all other opamps.