I was recently shown an article (several years old) in which a strain of bacteria in a very carefully controlled experiment arguably evolved into a new species. I'm not an expert on what does and does not constitute a species differentiation, but the gist of this result is that the bacteria were cultured in a nutrient deficient environment and they evolved the ability to metabolize a form of sugar that was present but that originally they couldn't metabolize. But the really big thing was that in order for this to happen, TWO separate adaptations had to happen in different parts of the DNA and either adaptation alone gave no increased survival benefit. So it appears to be an example of a random mutation being maintained in the DNA not because it is "good", but because it wasn't sufficiently "bad" to be selected against. So when the other random mutation occurred, NOW you had an organism that had a significant survival advantage over it's peers and so it flourished. I believe that the original strain became extinct (within the flasks that the superior strain evolved in).