Try this
View attachment 96861

I referred to your suggested circuit regarding instrumentation amplifier and actually I wanted to supply 33-409 mV to the input of it and wanted to get the amplified voltage in the range of 0-5 V, presently I constructed the following circuit which I am attaching with this post. Please tell me what changes shall I do to achieve so.

Note: This thread was split from:
https://forum.allaboutcircuits.com/threads/instrumentation-amplifier-problem.118670/#post-1142189

 

Hello,

You will need to subtract the 33 mV offset from the input.
The gain will be about 5000 / (409 - 33) = 13.3 times

Bertus