I use NIdaq where i can use FGEN, Osiloscope as software.

I cannot believe that result. Why isn't it work?

 

We will need more background to help you. Add...
- a better picture so we can read what your wires are connected to on your protoboard
- add a schematic (hand drawn or computer drawn so we know what your goal is
- briefly explain what the circuit is supposed to do

 

The function generator has 0Vpp. It also has 3VDC offset. So you will only have DC at AI1.

Is AI1 configured as single ended (ground referenced) or differential input?