Inhibitor effect on enzyme

[boks]

http://i35.tinypic.com/2pzy4xh.jpg

I'm asked to calculate Vmax and KM from this, with and without the inhibior, and answer what kind of inhibitor it is.

I have plotted 1/[S] vs 1/V0 here:

http://i36.tinypic.com/2ekrrz9.jpg

The x-intercept is -1/KM. The value I get is -1/KM=-4E4, so that KM=25E-6. Does this mean that KM=25E-6 M? My book says 20-24 M. Of course, I might have been unaccurate in drawing the graph, but why do I miss by a factor of 1E6?

[jpanhalt]

A Km of 24 molar is pretty high in my experience. By comparison, pure water is 55.5 molar. Are you sure the book's value is not the typo? I didn't do the calculations, but from the data in the table, a Km in the range of 10E-6 M seems more reasonable.

John